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. 2000 Feb;38(2):855-7.
doi: 10.1128/JCM.38.2.855-857.2000.

Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis

M K Taha  1
Affiliations

Simultaneous approach for nonculture PCR-based identification and serogroup prediction of Neisseria meningitidis

M K Taha. J Clin Microbiol. 2000 Feb.

Abstract

A nonculture PCR-based method to characterize Neisseria meningitidis was used to test 225 clinical specimens. PCR correctly identified and predicted the serogroups of N. meningitidis of culture-proven meningococcal diseases and confirmed this diagnosis in 35% of suspected samples. This approach could be useful when culture fails to isolate N. meningitidis.

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Figures

FIG. 1
FIG. 1
PCR amplification of the siaD (serogroups B, C, and Y and W135) and orf-2 (serogroup A) genes from strains belonging to serogroups A (strain LNP10824), B (strain LNP10846), C (strain LNP13331), and Y and W135 (strains LNP13145 and LNP13230, respectively) and from three clinical specimens (lanes 1, 2, and 3). The strains used were previously described (6). Lane − is the negative control (no bacteria). Electrophoresis was done on a 2% agarose gel. Size markers (bacteriophage φX174 digested by HaeIII) are indicated in base pairs at the right.
See this image and copyright information in PMC

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