Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mites
- PMID: 10668860
- DOI: 10.1023/a:1006251220052
Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mites
Abstract
Preliminary work is needed to assess the usefulness of different markers at different taxonomic scales when a new group is analyzed, such as the commercially important Phytoseiidae mites. We investigate here the level of sequence variation of the nuclear ribosomal spacers ITS 1 and 2 and the 5.8S gene in six species of Phytoseiidae: Neoseiulus culifornicus, N. fallacis, Euseius concordis, Metaseiulus occidentalis, Typhlodromus pyri and Phytoseiulus persimilis. As expected, the 5.8S gene (148 base pairs) is markedly conserved and displays little variation in between genera comparisons. ITS1 and ITS2 show contrasting patterns: while the ITS2 is short (80-89 bp) and shows little variation, the ITS1 is longer (303-404 bp) and is very variable in sequence. This fact compromises reliable nucleotide homologies when comparing the genera. The comparison of ITS1 sequence similarity at the species level might be useful for species identification, however, the value of ITS in taxonomic studies does not extend to the level of the family. The intraspecific variations of ITS were investigated in three species: N. californicus, N. fallacis and E. concordis. The first species has identical ITS1 sequences and the last two display low polymorphism (2 nucleotide substitutions). The ITS2 and 5.8S sequences were identical in all three subspecies comparisons.
Similar articles
-
Sequence analysis of the 5.8S ribosomal DNA and internal transcribed spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance.DNA Seq. 1996;6(6):361-4. doi: 10.3109/10425179609047576. DNA Seq. 1996. PMID: 8988376
-
Utility of ITS1-5.8S-ITS2 and 16S mitochondrial DNA sequences for species identification and phylogenetic inference within the Rhinonyssus coniventris species complex (Acari: Rhinonyssidae).Parasitol Res. 2007 Apr;100(5):1041-6. doi: 10.1007/s00436-006-0356-z. Epub 2006 Nov 10. Parasitol Res. 2007. PMID: 17096140
-
Species-wide homogeneity of nuclear ribosomal ITS2 sequences in the spider mite Tetranychus urticae contrasts with extensive mitochondrial COI polymorphism.Heredity (Edinb). 1998 Jun;80 ( Pt 6):742-52. doi: 10.1046/j.1365-2540.1998.00349.x. Heredity (Edinb). 1998. PMID: 9675873
-
Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions.Vet Res Commun. 2009 Oct;33(7):611-8. doi: 10.1007/s11259-009-9210-y. Epub 2009 Feb 13. Vet Res Commun. 2009. PMID: 19214768
-
The application of molecular markers in the study of diversity in acarology: a review.Exp Appl Acarol. 2000;24(10-11):751-74. doi: 10.1023/a:1006497906793. Exp Appl Acarol. 2000. PMID: 11345314 Review.
Cited by
-
Molecular identification of house dust mites and storage mites.Exp Appl Acarol. 2011 Oct;55(2):123-33. doi: 10.1007/s10493-011-9460-6. Epub 2011 Apr 6. Exp Appl Acarol. 2011. PMID: 21468750
-
First report and evidence of multiple origins of diflubenzuron resistance alleles in Culex pipiens mosquito from Cyprus.Parasit Vectors. 2025 Jun 20;18(1):231. doi: 10.1186/s13071-025-06889-7. Parasit Vectors. 2025. PMID: 40542391 Free PMC article.
-
Identification and detection of a novel point mutation in the Chitin Synthase gene of Culex pipiens associated with diflubenzuron resistance.PLoS Negl Trop Dis. 2020 May 1;14(5):e0008284. doi: 10.1371/journal.pntd.0008284. eCollection 2020 May. PLoS Negl Trop Dis. 2020. PMID: 32357192 Free PMC article.
-
Morphological, morphometric, and molecular characterization of Hepatozoon spp. (Apicomplexa, Hepatozoidae) from naturally infected Caudisona durissa terrifica (Serpentes, Viperidae).Parasitol Res. 2012 Apr;110(4):1393-401. doi: 10.1007/s00436-011-2639-2. Epub 2011 Sep 16. Parasitol Res. 2012. PMID: 21922238
-
Biological Control of Three Major Cucumber and Pepper Pests: Whiteflies, Thrips, and Spider Mites, in High Plastic Tunnels Using Two Local Phytoseiid Mites.Plants (Basel). 2024 Mar 20;13(6):889. doi: 10.3390/plants13060889. Plants (Basel). 2024. PMID: 38592899 Free PMC article.
References
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Research Materials