Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1999 Nov;23(11):851-9.
doi: 10.1023/a:1006251220052.

Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mites

Affiliations

Sequence variation of ribosomal internal transcribed spacers (ITS) in commercially important Phytoseiidae mites

M Navajas et al. Exp Appl Acarol. 1999 Nov.

Abstract

Preliminary work is needed to assess the usefulness of different markers at different taxonomic scales when a new group is analyzed, such as the commercially important Phytoseiidae mites. We investigate here the level of sequence variation of the nuclear ribosomal spacers ITS 1 and 2 and the 5.8S gene in six species of Phytoseiidae: Neoseiulus culifornicus, N. fallacis, Euseius concordis, Metaseiulus occidentalis, Typhlodromus pyri and Phytoseiulus persimilis. As expected, the 5.8S gene (148 base pairs) is markedly conserved and displays little variation in between genera comparisons. ITS1 and ITS2 show contrasting patterns: while the ITS2 is short (80-89 bp) and shows little variation, the ITS1 is longer (303-404 bp) and is very variable in sequence. This fact compromises reliable nucleotide homologies when comparing the genera. The comparison of ITS1 sequence similarity at the species level might be useful for species identification, however, the value of ITS in taxonomic studies does not extend to the level of the family. The intraspecific variations of ITS were investigated in three species: N. californicus, N. fallacis and E. concordis. The first species has identical ITS1 sequences and the last two display low polymorphism (2 nucleotide substitutions). The ITS2 and 5.8S sequences were identical in all three subspecies comparisons.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Q Rev Biol. 1991 Dec;66(4):411-53 - PubMed
    1. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10221-5 - PubMed
    1. Annu Rev Entomol. 1997;42:291-321 - PubMed
    1. Mol Ecol. 1995 Jun;4(3):383-7 - PubMed
    1. Insect Mol Biol. 1996 May;5(2):93-107 - PubMed

LinkOut - more resources