[Effect of apolipoprotein E gene plasma levels of lipids, lipoproteins, apolipoproteins and its relation with coronary heart disease]

Abstract

Using a technique of polymerase chain reaction-restriction lengths polymorphism (PCR-RLP), the authors detected the genotypes of apolipoprotein EE(ApoE) in 102 normal control subjects and in 118 patients with coronary heart disease (CHD). Meanwhile, the effect of ApoE allele on plasma lipid, lipoprotein, apolipoprotein levels were analyzed, and ApoE allele frequencies of patients with CHD and of control subjects were compared. The results showed that the technique of modified PCR-RFLP was a useful measure to detect the genotypes of ApoE. ApoE gene was polymorphism, resulting in plasma lipid level variance among individuals. As compared to ApoE3 allele, ApoE4 allele had positive effect on plasma TC, LDL-C and ApoB100 levels; while ApoE2 allele had negative effect on plasma TC, LDL-C and ApoB100 levels. The frequency of E4 allele in patients with CHD was higher than that in control subjects. The results in the present study suggest that ApoE4 allele is probably the genetic marker of CHD.