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. 1976 Oct;3(10):2485-90.
doi: 10.1093/nar/3.10.2485.

Construction of a hybrid col E1 plasmid carrying the gene for bacteriophage lambda repressor

L P Tikhomirova, A S Solonin, V N Ksenzenko, N I Matvienko
Free PMC article

Construction of a hybrid col E1 plasmid carrying the gene for bacteriophage lambda repressor

L P Tikhomirova et al. Nucleic Acids Res. 1976 Oct.
Free PMC article

Abstract

A biologically active hybrid DNA molecule was constructed from plasmid Col E1 and the Eco R1 fragment of lambda DNA containing the gene for lambda repressor. The presence of this gene in the hybrid molecule was demonstrated genetically. The hybrid plasmid contains two closely located targets for restriction endonuclease Hind 111 in the integrated fragment. Thus, the plasmid may be used as a vector not only for Eco R1 fragments but also for Hind 111 fragments.

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References

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