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. 1976 Nov;73(11):4174-8.
doi: 10.1073/pnas.73.11.4174.

Construction of plasmids carrying the cI gene of bacteriophage lambda

K Backman, M Ptashne, W Gilbert

Construction of plasmids carrying the cI gene of bacteriophage lambda

K Backman et al. Proc Natl Acad Sci U S A. 1976 Nov.

Abstract

By techniques of recombination in vitro, we have constructed a plasmid bearing the repressor gene (cI) of bacteriophage lambda fused to the promoter of the lac operon. Strains carrying this plasmid overproduce lambda repressor. This functional cI gene was reconstituted by joining DNA fragments bearing different parts of that gene. Flush end fusion techniques, involving no sequence overlap, were necessary for the construction; in certain cases, the abutting of the DNA molecules bearing ends generated by different restriction endonucleases creates a sequence at the junction which is recognized by one of the restriction endonucleases.

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References

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