Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Mar;66(3):1216-9.
doi: 10.1128/AEM.66.3.1216-1219.2000.

Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines

H V McNeill  1 , K A Sinha, C E Hormaeche, J J Lee, C M Khan
Affiliations

Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines

H V McNeill et al. Appl Environ Microbiol. 2000 Mar.

Abstract

We report the novel application of a herbicide-resistance-based dominant marker for the positive selection of expression plasmids in Salmonella serovar vaccines. The beta-lactamase gene of the plasmid pTETnir15, which expresses fragment C of tetanus toxin (TetC), has been replaced with the bar gene marker. The new plasmid pBAT1 can be positively selected in vitro within Salmonella serovars in the presence of the herbicide DL-phosphinothricin. The expression of TetC remains unaltered, and the Salmonella enterica serovar Typhimurium vaccine strain is stable and immunogenic in vivo.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Strategy for construction of pBAT1.
FIG. 2
FIG. 2
Immunoglobulin G antibody responses to recombinant TetC as detected by enzyme-linked immunosorbent assay from the pooled sera of mice immunized with C5HtrA(pTETnir15) and C5HtrA(pBAT1). Antibody responses were detected 3 weeks (A) and 5 weeks (B) postimmunization. OD, optical density.
See this image and copyright information in PMC

References

    1. Brown A, Hormaeche C E, Demarco de Hormaeche R, Dougan G, Winther M, Maskell D, Stocker B A D. An attenuated aroA S. typhimurium vaccine strain elicits humoral and cellular immunity to cloned β-galactosidase in mice. J Infect Dis. 1987;155:86–92. - PubMed
    1. Chatfield S N, Fairweather N F, Charles I, Pickard D, Levine M, Hone D, Posada M, Strugnell R A, Dougan G. Construction of a genetically defined Salmonella typhi Ty2 aroA aroC mutant for engineering of a candidate oral typhoid-tetanus vaccine. Infect Immun. 1992;10:53–60. - PubMed
    1. Chatfield S N, Charles I G, Makoff A J, Oxer M D, Dougan G, Slater D, Fairweather N F. Use of the nirB promoter to direct the stable expression of heterologous antigens in Salmonella oral vaccine strains: development of a single-dose oral vaccine. Bio/Technology. 1992;10:888–892. - PubMed
    1. Davis R W, Botstein D, Roth J R. A manual for genetic engineering: advanced bacterial genetics. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory; 1982.
    1. Gonzalez C, Hone D, Noriega F R, Tacket C O, Davis J R, Losonsky G, Nataro J P, Hoffman S, Malik A, Nardin E, Sztein B, Heppner D G, Fouts T R, Isibasi A, Levine M M. Salmonella typhi vaccine strain CVD 908 expressing the circumsporozoite protein of Plasmodium falciparum: strain construction and safety and immunogenicity in humans. J Infect Dis. 1994;169:927–931. - PubMed

Publication types