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. 2000 Mar;38(3):1144-50.
doi: 10.1128/JCM.38.3.1144-1150.2000.

Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay

D Aubert  1 , G T Maine, I Villena, J C Hunt, L Howard, M Sheu, S Brojanac, L E Chovan, S F Nowlan, J M Pinon
Affiliations

Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay

D Aubert et al. J Clin Microbiol. 2000 Mar.

Abstract

We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initial evaluation, six recombinant antigens (P29, P30, P35, P54, P66, and P68) were tested in the IgG and IgM Rec-ELISAs with four groups of samples which span the toxoplasmosis disease spectrum (negative, chronic infection, acute infection, and recent seroconversion). Our results suggest that the combination of P29, P30, and P35 in an IgG Rec-ELISA and the combination of P29, P35, and P66 in an IgM Rec-ELISA can replace the tachyzoite antigen in IgG and IgM serologic tests, respectively. The relative sensitivity, specificity, and agreement for the IgG P29-P30-P35 Rec-ELISA were 98.4, 95.7, and 97.2%, respectively. The resolved sensitivity, specificity, and agreement for the IgM P29-P35-P66 Rec-ELISA were 93.1, 95.0, and 94. 5%, respectively. Relative to the tachyzoite-based immunocapture IgM assay, the IgM P29-P35-P66 Rec-ELISA detects fewer samples that contain IgG antibodies with elevated avidity from individuals with an acute toxoplasmosis.

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Figures

FIG. 1
FIG. 1
Two representative examples of follow-up of women who experienced an acute toxoplasmosis. These women were followed by the T. gondii IgG P29-P30-P35 Rec-ELISA and IgM P29-P35-P66 Rec-ELISA (vertical bars, first y-axis), the HSDA (9) (indicated below the abscissa of each graph), and by the IC-M and IC-A assays (vertical bars, second y-axis). The cutoffs for the IgG P29-P30-P35 (A490, ≥0.65) and IgM P29-P35-P66 (A490, ≥0.51) Rec-ELISAs are indicated by the left arrow on the first y-axis. The cutoffs for the IC-M (index ≥6) and IC-A (index, ≥2) assays are indicated by the right arrows on the second y-axis as shown. The cutoff for the HSDA assay was 10 IU/ml (not indicated on graph). Toxo, T. gondii. IC, immunocapture.
See this image and copyright information in PMC

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