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Comparative Study
. 2000 Mar;38(3):1203-8.
doi: 10.1128/JCM.38.3.1203-1208.2000.

Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru

L Caviedes  1 , T S Lee, R H Gilman, P Sheen, E Spellman, E H Lee, D E Berg, S Montenegro-James
Affiliations
Comparative Study

Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru

L Caviedes et al. J Clin Microbiol. 2000 Mar.

Abstract

Inexpensive, rapid, and reliable methods of detecting infection by and drug susceptibility of Mycobacterium tuberculosis (MTB) are crucial to the control of tuberculosis. The novel microscopic observation broth-drug susceptibility assay (MODS) detects early growth of MTB in liquid medium, allowing more timely diagnosis and drug susceptibility testing. Sputum samples from hospitalized patients in Peru were analyzed by using stains, culture, and PCR. Sensitivity of MODS (92%) compared favorably with the most sensitive of the other culture methods (93%). Sputum samples positive for tuberculosis were tested for susceptibility to isoniazid and rifampin with the microwell alamar blue assay (MABA) and MODS. In 89% of cases, there was concordance between MODS and MABA. Of the diagnostic and susceptibility testing methods used, MODS yielded results most rapidly (median, 9.0 and 9.5 days, respectively). MODS is a rapid, inexpensive, sensitive, and specific method for MTB detection and susceptibility testing; it is particularly appropriate for use in developing countries burdened by significant infection rates and increasing numbers of multiple-drug-resistant cases.

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Figures

FIG. 1
FIG. 1
Appearance of M. tuberculosis at 100× magnification after 25 days of culture in broth. Note the serpentine, ropy aspect of the microcolonies.
See this image and copyright information in PMC

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