Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Mar;38(3):1298-301.
doi: 10.1128/JCM.38.3.1298-1301.2000.

Genetic and antigenic evidence supports the separation of Hepatozoon canis and Hepatozoon americanum at the species level

G Baneth  1 , J R Barta, V Shkap, D S Martin, D K Macintire, N Vincent-Johnson
Affiliations

Genetic and antigenic evidence supports the separation of Hepatozoon canis and Hepatozoon americanum at the species level

G Baneth et al. J Clin Microbiol. 2000 Mar.

Abstract

Recognition of Hepatozoon canis and Hepatozoon americanum as distinct species was supported by the results of Western immunoblotting of canine anti-H. canis and anti-H. americanum sera against H. canis gamonts. Sequence analysis of 368 bases near the 3' end of the 18S rRNA gene from each species revealed a pairwise difference of 13.59%.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
Alignment of a portion of the 18S rRNA gene from H. americanum (United States) and H. canis (Israel) and two Hepatozoon species, H. sipedon from a snake and H. catesbianae from a frog. Only the nucleotides that are different from those of H. sipedon are shown.
FIG. 2
FIG. 2
Western blot analysis of H. canis gamont antigens recognized by sera from dogs infected with H. canis and H. americanum. Reactions of sera from different dogs are shown as follows: lane 1, serum from a dog experimentally infected with H. canis; lane 2, serum from a dog with a naturally occurring H. canis infection; lanes 3 to 5, sera from three dogs that were naturally infected with H. americanum; lanes 6 and 7, control serum from a blood donor dog at Auburn University and from preinfection serum from the experimentally infected dog, respectively. Lane M contains molecular mass markers. Note the triplet of bands with relative rMM of 32 to 28 kDa that are reactive only with anti-H. canis sera.
See this image and copyright information in PMC

References

    1. Baneth G, Weigler B. Retrospective case-control study of hepatozoonosis in dogs in Israel. J Vet Intern Med. 1997;11:365–370. - PubMed
    1. Baneth G, Shkap V, Samish M, Pipano E, Savitsky I. Antibody response to Hepatozoon canis in experimentally infected dogs. Vet Parasitol. 1998;74:299–305. - PubMed
    1. Barta J R, Martin D S, Liberator P A, Dashkevicz M, Anderson J W, Feighner S D, Elbrecht A, Perkins-Barrow A, Jenkins M C, Danforth H D, Ruff M D, Profous-Juchelka H. Phylogenetic relationships among eight Eimeria species infecting the domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol. 1997;83:262–271. - PubMed
    1. Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–254. - PubMed
    1. Christophers S R. The sexual life cycle of Leucocytozoon canis in the tick. Sci Mem Off Med Sanit Dep Gov India. 1907;28:1–11.

LinkOut - more resources