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. 2000 Mar 24;470(2):151-5.
doi: 10.1016/s0014-5793(00)01313-2.

Polymyxin B binds to anandamide and inhibits its cytotoxic effect

Y Wang  1 , Y Liu, K P Sarker, M Nakashima, T Serizawa, A Kishida, M Akashi, M Nakata, I Kitajima, I Maruyama
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Free article

Polymyxin B binds to anandamide and inhibits its cytotoxic effect

Y Wang et al. FEBS Lett. .
Free article

Abstract

Anandamide (ANA), an endogenous cannabinoid, can be generated by activated macrophages during endotoxin shock and is thought to be a paracrine contributor to hypotension. We discovered that ANA in saline/ethanol solution and in serum was efficiently adsorbed in a polymyxin B (PMB)-immobilized beads column and eluted with ethanol. We confirmed the direct binding of PMB to ANA by using surface plasmon resonance. The adsorption of ANA by PMB may abolish the diverse effects of ANA such as hypotension, immunosuppression, and cytotoxicity, and may suggest a new therapeutic strategy for endotoxin shock.

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