Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jan 5;168(1):111-5.
doi: 10.1007/BF00267940.

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA

S Chang, S N Cohen

High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA

S Chang et al. Mol Gen Genet. .

Abstract

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) transformants per microgram of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1433-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 - PubMed
    1. J Bacteriol. 1975 Jun;122(3):987-93 - PubMed
    1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1974;14(0):39-100 - PubMed

LinkOut - more resources