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. 2000 Apr;38(4):1632-5.
doi: 10.1128/JCM.38.4.1632-1635.2000.

Characterization of a recurrent clonal type of Escherichia coli O157:H7 causing major outbreaks of infection in Scotland

L J Allison  1 , P E Carter, F M Thomson-Carter
Affiliations

Characterization of a recurrent clonal type of Escherichia coli O157:H7 causing major outbreaks of infection in Scotland

L J Allison et al. J Clin Microbiol. 2000 Apr.

Abstract

A particular recurrent clonal type of Escherichia coli O157 has been isolated from multiple clinical, veterinary, food, and environmental sources throughout Scotland since 1989. Significant genotypic variation was detected among isolates from distinct outbreaks, with the presence or absence of single fragments being sufficient to delineate outbreak groups within the clonal type.

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Figures

FIG. 1
FIG. 1
PFGE profiles of genomic DNAs West Lothian, Highland, and central Scotland outbreak isolates cleaved with XbaI (lanes 2 to 4), XhoI (lanes 5 to 7), NotI (lanes 8 to 10), and SfiI (lanes 11 to 13). Lanes 1 and 14, bacteriophage lambda concatemer size markers. The unnumbered lanes correspond to lanes 1 to 14 from left to right, respectively.
FIG. 2
FIG. 2
PFGE profiles of AvrII-cleaved genomic DNAs from West Lothian (lanes 1 to 3), Highland (lanes 4 and 5), and central Scotland (lanes 6 to 9) outbreak isolates. Lane 10, bacteriophage lambda concatemer size markers. The fragment identifying central Scotland isolates is indicated by A. The fragment absent from central Scotland isolates but present in both West Lothian and Highland isolates is indicated by B. The unnumbered lanes correspond to lanes 1 to 10 from left to right, respectively.
FIG. 3
FIG. 3
PFGE profiles of XbaI-cleaved genomic DNA from West Lothian (lanes 2 to 4), Highland (lanes 5 to 7), and central Scotland (lanes 8 to 10) outbreak isolates. Lanes 1 and 11, bacteriophage lambda concatemer size markers. The unnumbered lanes correspond to lanes 1 to 11 from left to right, respectively.
FIG. 4
FIG. 4
Bacteriophage lambda RFLP analysis of PvuII-cleaved genomic DNA from West Lothian (lanes 2 to 10, and 18), Highland (lane 11), and central Scotland (lanes 12 to 17) outbreak isolates. Lanes 1 and 19, DNA molecular weight standards. The unnumbered lanes correspond to lanes 1 to 19 from left to right, respectively.
See this image and copyright information in PMC

References

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