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. 2000 Mar;30(3):178-82.
doi: 10.1046/j.1472-765x.2000.00675.x.

A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria

J McKerrow  1 , S Vagg, T McKinney, E M Seviour, A M Maszenan, P Brooks, R J Seviour
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A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria

J McKerrow et al. Lett Appl Microbiol. 2000 Mar.

Abstract

A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required.

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