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. 2000 Mar;33(2):103-6.
doi: 10.1016/s0009-9120(99)00090-9.

Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals

S Tsakiris  1 , P Angelogianni, K H Schulpis, J C Stavridis
Affiliations

Protective effect of L-phenylalanine on rat brain acetylcholinesterase inhibition induced by free radicals

S Tsakiris et al. Clin Biochem. 2000 Mar.

Abstract

Objective: To investigate whether the preincubation of brain homogenates with L-phenylalanine (Phe) could reverse the free radical effects on brain acetylcholinesterase (AChE) activity, since it has been reported that Phe binds hydroxyl radicals ((*)OH).

Design and methods: Two well established systems were used for production of free radicals: (a) FeSO(4) (84 microM) plus ascorbic acid (400 microM), and (b) FeSO(4), ascorbic acid and H(2)O(2) (1 mM) at 37 degrees C in homogenates of adult rat whole brain. Changes in brain AChE activity were studied in the presence of each system separately.

Results: AChE was inhibited (18-28%) by both systems of free radicals. This inhibition was reversed when the brain homogenate was preincubated with Phe 1.8 mM.

Conclusions: In accordance with our previous reports, Phe could protect against the direct action of (*)OH radicals on brain AChE and in this way it might be useful in the prevention of certain cholinergic neural dysfunctions.

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