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. 2000 Mar;9(3):435-9.
doi: 10.1110/ps.9.3.435.

Detection of intact megaDalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry

W J van Berkel  1 , R H van den Heuvel, C Versluis, A J Heck
Affiliations

Detection of intact megaDalton protein assemblies of vanillyl-alcohol oxidase by mass spectrometry

W J van Berkel et al. Protein Sci. 2000 Mar.

Abstract

Well-resolved ion signals of intact large protein assemblies, with molecular masses extending above one million Dalton, have been detected and mass analyzed using electrospray ionization mass spectrometry, with an uncertainty in mass of <0.2%. The mass spectral data seem to reflect known solution-phase behavior of the studied protein assembly and have therefore been directly used to probe the protein assembly topology and stability as a function of ionic strength and pH.

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