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. 2000 Jan;17(1):67-73.
doi: 10.1023/a:1009458215882.

Study of the in vitro maturation of mouse oocytes induced by microinjection of maturation promoting factor (MPF)

H Nakano  1 , H Kubo
Affiliations

Study of the in vitro maturation of mouse oocytes induced by microinjection of maturation promoting factor (MPF)

H Nakano et al. J Assist Reprod Genet. 2000 Jan.

Abstract

Purpose: Maturation promoting factor (MPF) acts at the resumption of meiosis and nonspecifically throughout the animal species. There exists a considerable body of literature on MPF, but little work has been done to study the induction of maturation of mammalian oocytes by microinjection of extracted MPF.

Methods: Immature (GV-stage) mouse oocytes were microinjected MPF extracted from matured Xenopus eggs in the presence of dbcAMP.

Results: The rate of germinal vesicle, breakdown (GVBD) induced at 24 hr after MPF injection was significantly higher (90.5%) than that of the control (2.2%), which was injected with HTF medium containing dbcAMP (P < 0.0001). The rate of extrusion of the first polar body at 24 hr after MPF injection was significantly higher (84.1%) than that of the same control (1.1%) (P < 0.0001).

Conclusions: From these results, it is concluded that the maturation of mammalian oocytes can be induced by the microinjection of MPF extracted from other species.

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