Simple dipstick assay for semi-quantitative detection of neopterin in sera
- PMID: 10758235
- DOI: 10.1016/s0022-1759(00)00148-4
Simple dipstick assay for semi-quantitative detection of neopterin in sera
Abstract
Neopterin, a low-molecular-mass pteridine produced by macrophages, is closely associated with activation of the cellular immune system. Neopterin biosynthesis during inflammatory disease is primarily derived from interferon-activated monocytes/macrophages and neopterin concentrations may be significantly increased in a particular disease state compared to controls. A follow-up of serum neopterin concentrations during the course of an infectious disease could be useful for measuring the activity of the disease and the influence of treatment. We have developed a simple dipstick assay for the semi-quantitative detection of the neopterin concentration in the serum of patients during the course of an infectious disease. Assay performance was comparable to an ELISA, but there is no requirement for specialised equipment.
Similar articles
-
Evaluation of a new enzyme-linked immunosorbent assay for the determination of neopterin.Clin Lab. 2005;51(9-10):495-504. Clin Lab. 2005. PMID: 16285471
-
Evaluation of a new simple and rapid enzyme-linked immunosorbent assay kit for neopterin determination.Clin Chem Lab Med. 2000 Apr;38(4):345-53. doi: 10.1515/CCLM.2000.050. Clin Chem Lab Med. 2000. PMID: 10928656
-
Association of increased neopterin production with decreased humoral immunity in the elderly.Exp Gerontol. 2003 May;38(5):583-7. doi: 10.1016/s0531-5565(03)00062-7. Exp Gerontol. 2003. PMID: 12742536
-
Neopterin as a marker of cellular immunological response.Postepy Hig Med Dosw (Online). 2017 Aug 24;71(1):727-736. doi: 10.5604/01.3001.0010.3851. Postepy Hig Med Dosw (Online). 2017. PMID: 28894045 Review.
-
Potential role of immune system activation-associated production of neopterin derivatives in humans.Inflamm Res. 2003 Aug;52(8):313-21. doi: 10.1007/s00011-003-1181-9. Inflamm Res. 2003. PMID: 14504669 Review.
Cited by
-
Determination of Urinary Neopterin/Creatinine Ratio to Distinguish Active Tuberculosis from Latent Mycobacterium tuberculosis Infection.J Biomark. 2016;2016:5643853. doi: 10.1155/2016/5643853. Epub 2016 Jun 28. J Biomark. 2016. PMID: 27433370 Free PMC article.
-
Serum neopterin is elevated in patients infected with Shigella.Gut Pathog. 2010 Aug 22;2(1):9. doi: 10.1186/1757-4749-2-9. Gut Pathog. 2010. PMID: 20727206 Free PMC article.
-
Neopterin in Diagnosis and Monitoring of Infectious Diseases.J Biomark. 2013;2013:196432. doi: 10.1155/2013/196432. Epub 2013 Dec 8. J Biomark. 2013. PMID: 26317013 Free PMC article. Review.
-
Cerebrospinal fluid neopterin as marker of the meningo-encephalitic stage of Trypanosoma brucei gambiense sleeping sickness.PLoS One. 2012;7(7):e40909. doi: 10.1371/journal.pone.0040909. Epub 2012 Jul 18. PLoS One. 2012. PMID: 22815865 Free PMC article.
-
Enabling robust quantitative readout in an equipment-free model of device development.Analyst. 2014 Oct 7;139(19):4750-7. doi: 10.1039/c4an01003e. Analyst. 2014. PMID: 25089298 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources