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. 2000 Apr 4;246(1-2):179-85.
doi: 10.1016/s0378-1119(00)00083-4.

A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

A De Antoni  1 , D Gallwitz
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A novel multi-purpose cassette for repeated integrative epitope tagging of genes in Saccharomyces cerevisiae

A De Antoni et al. Gene. .

Abstract

Gene tagging can be achieved by homologous recombination in yeast. The kan(r) marker gene plays an important role in PCR-mediated gene disruption and PCR-mediated epitope tagging experiments. In this paper, new modules containing a tag-loxP-kanMX-loxP cassette are described that allow tagging of different genes by using the kan(r) marker repeatedly.

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