Solvent-isotope and pH effects on flagellar rotation in Escherichia coli

Abstract

We studied changes in speed of the flagellar rotary motor of Escherichia coli when tethered cells or cells carrying small latex spheres on flagellar stubs were shifted from H(2)O to D(2)O or subjected to changes in external pH. In the high-torque, low-speed regime, solvent isotope effects were found to be small; in the low-torque, high-speed regime, they were large. The boundaries between these regimes were close to those found earlier in measurements of the torque-speed relationship of the flagellar rotary motor (, Biophys. J. 65:2201-2216;, Biophys. J., 78:1036-1041). This observation provides direct evidence that the decline in torque at high speed is due primarily to limits in rates of proton transfer. However, variations of speed (and torque) with shifts of external pH (from 4.7 to 8.8) were small for both regimes. Therefore, rates of proton transfer are not very dependent on external pH.