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. 2000 Mar;36(3):309-13.

[Transformation of Mycoplasma hominis by plasmid pAM120 using electroporation]

[Article in Russian]
N M Aleksandrova  1 , M R Bevova, V M Govorun
Affiliations
  • PMID: 10779903

[Transformation of Mycoplasma hominis by plasmid pAM120 using electroporation]

[Article in Russian]
N M Aleksandrova et al. Genetika. 2000 Mar.

Abstract

Mycoplasma hominis was transformed by electroporation with plasmid pAM120 containing the transposon Tn916 that carried the tetM gene responsible for the resistance to tetracycline. The frequency of transformation was 10(-7)-10(-8) colony-forming units (CFU) per 10 micrograms of plasmid DNA. The PCR analysis of transformed DNA confirmed the transposon integration into the mycoplasma genome.

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