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. 2000 May;38(5):1823-6.
doi: 10.1128/JCM.38.5.1823-1826.2000.

Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections

D A Martin  1 , D A Muth, T Brown, A J Johnson, N Karabatsos, J T Roehrig
Affiliations

Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections

D A Martin et al. J Clin Microbiol. 2000 May.

Abstract

Immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) is a rapid and versatile diagnostic method that readily permits the combination of multiple assays. Test consolidation is especially important for arthropod-borne viruses (arboviruses) which belong to at least three virus families: the Togaviridae, Flaviviridae, and Bunyaviridae. Using prototype viruses from each of these families and a panel of well-characterized human sera, we have evaluated and standardized a combined MAC-ELISA capable of identifying virus infections caused by members of each virus family. Furthermore, by grouping antigens geographically and utilizing known serological cross-reactivities, we have reduced the number of antigens necessary for testing, while maintaining adequate detection sensitivity. We have determined that a 1:400 serum dilution is most appropriate for screening antiviral antibody, using a positive-to-negative ratio of >/=2.0 as a positive cutoff value. With a blind-coded human serum panel, this combined MAC-ELISA was shown to have test sensitivity and specificity that correlated well with those of other serological techniques.

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Figures

FIG. 1
FIG. 1
MAC-ELISA titration curves. Log10 serum dilution versus A450 is presented. Representative specimens of high-titered sera (■), medium-titered sera (●), low-titered sera (▴), and negative sera (⧫) are plotted.
See this image and copyright information in PMC

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