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. 1974 May;1(5):707-18.
doi: 10.1093/nar/1.5.707.

Transfer RNA methylating activity of yeast mitochondria

N Smolar, I Svensson
Free PMC article

Transfer RNA methylating activity of yeast mitochondria

N Smolar et al. Nucleic Acids Res. 1974 May.
Free PMC article

Abstract

Mitochondria isolated from Saccharomyces cerevisiae and purified in Urografin or sucrose gradient contain tRNA methylating activity with specificities different from those of the cytoplasm. The main reaction product, using E.coli tRNA as methyl group acceptor, is N(2),-N(2)-dimethylguanine. The corresponding mitochondrial methylase is coded by nuclear DNA. A DNA methylating activity is also associated with yeast mitochondria.

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