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. 1974 Jun;1(6):787-801.
doi: 10.1093/nar/1.6.787.

Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease

J N Hansen, B H Pheiffer, C J Hough
Free PMC article

Hybrid isolation by recovery of RNA-DNA hybrids from agar using S1 nuclease

J N Hansen et al. Nucleic Acids Res. 1974 Jun.
Free PMC article

Abstract

A method for recovering RNA-DNA hybrids from agar employing a single strand specific nuclease is described. The procedure is suitable for large scale isolations, and immobilization of the DNA in agar prior to hybridization allows a high yield of hybrid without interference by DNA reannealing.

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References

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