Studies on bacteriophage M13 DNA. 1. A cleavage map of the M13 genome

Abstract

A physical map of the bacteriophage M13 genome has been constructed on the basis of specific cleavage of M13 replicative form DNA by bacterial restriction endonucleases. The 13 fragments produced by the enzyme from Haemophilus aphirophilus (endonuclease R.Hap II) as well as the 10 fragments produced by the enzyme from Haemophilus aegyptius (endonuclease R.Hae III) have been ordered by analysis of partial digest products and by analysis of overlapping sets of fragments. In addition, the single site in M13 replicative form DNA cleaved by the restriction enzyme from Haemophilus influenzae Rd (endonuclease R.Hin dII) has been located more precisely. With this unique site as a reference point, the H. aphirophilus cleavage sites and the H. aegyptius cleavage sites have been localized on the map.