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. 2000 May;7(3):501-3.
doi: 10.1128/CDLI.7.3.501-503.2000.

Intestinal secretory immunoglobulin A response to enteroaggregative Escherichia coli in travelers with diarrhea

M Sutjita  1 , A R Bouckenooghe, J A Adachi, Z D Jiang, J J Mathewson, C D Ericsson, H L DuPont
Affiliations

Intestinal secretory immunoglobulin A response to enteroaggregative Escherichia coli in travelers with diarrhea

M Sutjita et al. Clin Diagn Lab Immunol. 2000 May.

Abstract

We examined stool samples from travelers for secretory immunoglobulin A (sIgA) to enteroaggregative Escherichia coli (EAEC) during episodes of acute diarrhea. Ten paired samples from 10 patients with diarrhea caused by EAEC were examined for the presence of specific sIgA by dot blot and Western blot immunoassays. Five samples were positive by dot blotting, and two samples were positive by Western blotting.

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Figures

FIG. 1
FIG. 1
Example of the Western blot results. Lane M, marker (numbers on the left are in kilodaltons); lane 1, reactivity of sample 37034p to crude extracts of strains 37054; the sample reacts mainly with an approximately 15-kDa antigen; lane 2, sample 47087p, which reacts to approximately 20-, 45-, and 85-kDa antigens; lane 3 and lane 4, sample 37067p and a stool sample from a healthy individual, respectively, which show no reactivity to crude extracts of EAEC.
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