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. 1975 Jun;122(3):1091-102.
doi: 10.1128/jb.122.3.1091-1102.1975.

Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae

J E LeClerc, J K Setlow

Single-strand regions in the deoxyribonucleic acid of competent Haemophilus influenzae

J E LeClerc et al. J Bacteriol. 1975 Jun.

Abstract

The deoxyribonucleic acid (DNA) of competent wild-type Haemophilus influenzae and rec1 mutant cells contains single-strand regions, as judged by alkaline sucrose sedimentation, benzoylated naphthoylated diethylaminoethyl-cellulose fractionation, and digestion with an enzyme specific for single-strand regions in DNA. In contrast, the DNA of competent rec2 cells does not contain single-strand regions. Since transforming DNA does not associate with recipient DNA in the rec2 mutant as it does in wild type and rec1, it is concluded that the single-strand regions in the DNA of the competent cells are important for an early step in recombination between cell DNA and transforming DNA.

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