Review
doi: 10.1007/978-3-0348-8458-7_12.
Lipopeptide preparation and analysis
Affiliations
- PMID: 10803379
- DOI: 10.1007/978-3-0348-8458-7_12
Item in Clipboard
Review
Lipopeptide preparation and analysis
EXS.
2000.
Abstract
Lipophilic peptides and proteins present specific problems during preparation and analysis which require the use of modified methodology. This chapter discusses some of the methods that have been employed in the isolation and structural studies of the pulmonary surfactant-associated proteins B and C (SP-B and SP-C), other proteins with lipid-like physicochemical properties, and the SP-B precursor. In particular, methods for separation and analysis of peptide/lipid mixtures, high-resolution separation of lipopeptides, analysis of fatty acylated peptides, and secondary and tertiary structure analysis of lipopeptides are discussed.
Similar articles
-
Method of purification affects some interfacial properties of pulmonary surfactant proteins B and C and their mixtures with dipalmitoylphosphatidylcholine.Biochim Biophys Acta. 1998 Mar 6;1370(1):138-50. doi: 10.1016/s0005-2736(97)00257-5. Biochim Biophys Acta. 1998. PMID: 9518582
-
Biophysical activity of an artificial surfactant containing an analogue of surfactant protein (SP)-C and native SP-B.Biochem J. 1999 Apr 15;339 ( Pt 2)(Pt 2):381-6. Biochem J. 1999. PMID: 10191270 Free PMC article.
-
Canine hydrophobic surfactant polypeptide SP-C. A lipopeptide with one thioester-linked palmitoyl group.FEBS Lett. 1991 Apr 9;281(1-2):119-22. doi: 10.1016/0014-5793(91)80373-b. FEBS Lett. 1991. PMID: 2015882
-
Palmitoylation and processing of the lipopeptide surfactant protein C.Biochim Biophys Acta. 2002 Aug 8;1583(3):253-65. doi: 10.1016/s1388-1981(02)00248-2. Biochim Biophys Acta. 2002. PMID: 12176392 Review.
-
Membrane properties and amyloid fibril formation of lung surfactant protein C.Biochem Soc Trans. 2001 Aug;29(Pt 4):601-6. doi: 10.1042/bst0290601. Biochem Soc Trans. 2001. PMID: 11498036 Review.