[Detection methods for drug-resistant bacteria in routine examination--MRSA]

Abstract

Since methicillin-resistant Staphylococcus aureus(MRSA) is resistant not only to methicillin but also to multiple species of antibacterial drugs, there are cases where infections with MRSA are difficult to treat. For laboratory identification of MRSA, S. aureus is first identified, then an oxacillin (MPIPC) sensitivity test, PCR detection of the S. aureus mecA gene and an agglutination test using the latex sensitized with an anti-PBP-2' monoclonal antibody are usually utilized. However, the detection of MRSA does not necessarily indicate MRSA infection, and many cases only demonstrate MRSA colonization. Thus, a clinical investigation is indispensable for establishing the diagnosis of MRSA infection. In addition, recent reports also suggested a current trend toward decreasing sensitivity to Bactroban(MUP, mupirocin, an anti-intranasal MRSA antibacterial drug). Taking these into consideration, surveillance of the trend in patient's sensitive to various species of antibacterial drugs including MUP must continue further.