Nonylphenol affects the granulation pattern of epidermal mucous cells in rainbow trout, Oncorhynchus mykiss

Abstract

Nonylphenol is a biodegradation product of nonionic surfactants and has recently attracted considerable attention due to its estrogenic potential. Sexually mature male rainbow trout were repeatedly exposed (one to four periods of 10 days each) to environmentally relevant concentrations of nonylphenol (1 microg/L, 10 microg/L) and for comparison, trout were injected with estradiol. Since estrogens are known to induce structural changes within the fish skin, a similar effect of xenobiotics with estrogen-like activity was assumed. Samples of skin were evaluated by means of light and electron microscopy and histochemistry. In trout exposed to nonylphenol and to estradiol, the structure of the epidermis was altered: an irregular overall architecture was often accompanied by detached pavement cells, vacuolation of the cytoplasm, and severely deformed cell nuclei. However, the granulation pattern of the mucous cells was influenced exclusively after exposition to nonylphenol. The number of large and irregularly shaped mucosomes depended more on the exposure period than on the concentration of nonylphenol. Furthermore, this alteration has not yet been reported for any other pollutant or stressor and, thus, can be classified as an effect that would strongly indicate exposure to nonylphenol.