Storage and metabolism of poly(adenylic acid)-mRNA in germinating cotton seeds
- PMID: 1080673
- DOI: 10.1021/bi00691a005
Storage and metabolism of poly(adenylic acid)-mRNA in germinating cotton seeds
Abstract
When the RNA from both the nucleus and polysomes of germinated cotton seeds were fractionated on poly(U)-cellulose columns it was found that both contained poly(A)-RNA. In ungerminated seeds most of the poly(A)-RNA is located in the nucleus of the cell. 3'-Deoxyadenosine was found to inhibit the synthesis of both nuclear and polysomal poly(A) by 75% in germinated seeds, but had little or no effect on protein synthesis during the first 6 hr of germination. After this time 3'-deoxyadenosine had a pronounced inhibitory effect on protein synthesis presumably due to the inhibition of poly(A) addition to newly synthesized mRNA.
Similar articles
-
Cotton messenger RNA sequences exist in both polyadenylated and nonpolyadenylated forms.J Biol Chem. 1981 Mar 10;256(5):2551-60. J Biol Chem. 1981. PMID: 7462254
-
Isolation and characterization of poly(adenylic acid)-containing messenger ribonucleic acid from rat liver polysomes.Biochim Biophys Acta. 1978 Jun 22;519(1):173-93. doi: 10.1016/0005-2787(78)90071-0. Biochim Biophys Acta. 1978. PMID: 667061
-
Molecular aspects of legume seed storage protein synthesis.Crit Rev Food Sci Nutr. 1982;16(2):187-98. doi: 10.1080/10408398209527333. Crit Rev Food Sci Nutr. 1982. PMID: 6121684 Review. No abstract available.
-
Polyadenylylation of stored mRNA in cotton seed germination.Prog Nucleic Acid Res Mol Biol. 1976;19:113-8. doi: 10.1016/s0079-6603(08)60912-5. Prog Nucleic Acid Res Mol Biol. 1976. PMID: 1087982 No abstract available.
-
Characteristics and significance of the polyadenylate sequence in mammalian messenger RNA.Prog Nucleic Acid Res Mol Biol. 1976;17:117-48. doi: 10.1016/s0079-6603(08)60068-9. Prog Nucleic Acid Res Mol Biol. 1976. PMID: 778919 Review. No abstract available.
Cited by
-
A model for cytoplasm-governed gene regulation.Biochem J. 1976 Dec 1;159(3):783-94. doi: 10.1042/bj1590783. Biochem J. 1976. PMID: 795425 Free PMC article. Review.
-
Deep sequencing of RNA from ancient maize kernels.PLoS One. 2013;8(1):e50961. doi: 10.1371/journal.pone.0050961. Epub 2013 Jan 11. PLoS One. 2013. PMID: 23326310 Free PMC article.
-
Disappearance of stored polyadenylic acid and mRNA during early germination of radish (Raphanus sativus L.) embryo axes.Planta. 1977 Jan;135(2):125-8. doi: 10.1007/BF00387160. Planta. 1977. PMID: 24420013
-
From the Outside to the Inside: New Insights on the Main Factors That Guide Seed Dormancy and Germination.Genes (Basel). 2020 Dec 31;12(1):52. doi: 10.3390/genes12010052. Genes (Basel). 2020. PMID: 33396410 Free PMC article. Review.
-
Messenger RNA is conserved during drying of the drought-tolerant moss Tortula ruralis.Proc Natl Acad Sci U S A. 1978 Feb;75(2):842-6. doi: 10.1073/pnas.75.2.842. Proc Natl Acad Sci U S A. 1978. PMID: 16592498 Free PMC article.