Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 May 4;2(9):1341-4.
doi: 10.1021/ol005831o.

Recognition of the nonpolar base 4-methylindole in DNA by the DNA repair adenine glycosylase MutY

C L Chepanoske  1 , C R Langelier, N H Chmiel, S S David
Affiliations

Recognition of the nonpolar base 4-methylindole in DNA by the DNA repair adenine glycosylase MutY

C L Chepanoske et al. Org Lett. .

Abstract

[formula: see text] The DNA repair adenine glycosylase MutY efficiently recognizes 7-deaza-2'-deoxyadenosine (Z) and its nonpolar isostere 4-methylindole beta-deoxynucleoside (M) opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) and G in DNA. Both wild-type and truncated MutY exhibit a 10- to 20-fold higher affinity for a duplex containing OG:M than OG:Z. More efficient recognition of M over Z by MutY may be to due the lack of hydrogen bonding with the OG that facilitates nucleotide flipping during the substrate recognition process.

PubMed Disclaimer

Publication types