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. 2000 Jun;44(6):1701-4.
doi: 10.1128/AAC.44.6.1701-1704.2000.

Characterization of a Mycobacterium smegmatis mutant that is simultaneously resistant to D-cycloserine and vancomycin

M Peteroy  1 , A Severin, F Zhao, D Rosner, U Lopatin, H Scherman, A Belanger, B Harvey, G F Hatfull, P J Brennan, N D Connell
Affiliations

Characterization of a Mycobacterium smegmatis mutant that is simultaneously resistant to D-cycloserine and vancomycin

M Peteroy et al. Antimicrob Agents Chemother. 2000 Jun.

Abstract

A mutant of Mycobacterium smegmatis has been isolated that is simultaneously resistant to both D-cycloserine (D-CS) and vancomycin. Genetic complementation with a PBP4 homolog restores sensitivity to both drugs. Resistance to D-CS and vancomycin in this mutant is most likely due to a novel mechanism involving peptidoglycan assembly at the cell surface.

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Figures

FIG. 1
FIG. 1
Vancomycin removal assay. Removal of vancomycin from the supernatant by M. smegmatis mc2155 (circles) or Cvr-1 (triangles) was monitored at 282 nm at the indicated time points. At each time point, the A282 was converted to micrograms of vancomycin per milliliter remaining in the supernatant. The data are the averages of three experiments performed in duplicate. Error bars are smaller than data points.
FIG. 2
FIG. 2
Amino acid sequence comparison of the complementing ORF with PBP4 from E. coli. The putative N-terminal signal sequence is underlined, and the active-site residue is indicated by the asterisk (see text). Identical residues are highlighted in darker boxes, whereas similar residues are highlighted in lighter boxes.
See this image and copyright information in PMC

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