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. 2000 Jun;44(6):1713-5.
doi: 10.1128/AAC.44.6.1713-1715.2000.

Mutations at four distinct regions of the rpoB gene can reduce the susceptibility of Helicobacter pylori to rifamycins

M Heep  1 , S Odenbreit, D Beck, J Decker, E Prohaska, U Rieger, N Lehn
Affiliations

Mutations at four distinct regions of the rpoB gene can reduce the susceptibility of Helicobacter pylori to rifamycins

M Heep et al. Antimicrob Agents Chemother. 2000 Jun.

Abstract

The transformation of a panel of rpoB mutations (codons 525 to 586) from rifampin-resistant donor strains into Helicobacter pylori 2802A confirmed the MICs associated with the respective mutations. RpoB V149X random mutations were generated and induced different levels of resistance, depending on the replacement amino acid. Mutagenesis of the rpoB gene at codon position 701 (R701H) induced low-level resistance.

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Comment in

  • Update on rifampin resistance in the Legionellaceae.
    Serr A, Koenig BF, Heep M, Nielsen K, Bangsborg JM. Serr A, et al. Antimicrob Agents Chemother. 2001 Jul;45(7):2181-2. doi: 10.1128/AAC.45.7.2181-2182.2001. Antimicrob Agents Chemother. 2001. PMID: 11441826 Free PMC article. No abstract available.

References

    1. Akada J K, Shirai M, Fujii K, Okita K, Nakazawa T. In vitro anti-Helicobacter pylori activities of new rifamycin derivatives, KRM-1648 and KRM-1657. Antimicrob Agents Chemother. 1999;43:1072–1076. - PMC - PubMed
    1. Heep M, Beck D, Bayerdörffer E, Lehn N. Rifampin and rifabutin resistance mechanism in Helicobacter pylori. Antimicrob Agents Chemother. 1999;43:1497–1499. - PMC - PubMed
    1. Heep M, Rieger U, Beck D, Lehn N. Mutations in the beginning of the rpoB gene can induce resistance to rifamycins in both Helicobacter pylori and Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2000;44:1075–1077. - PMC - PubMed
    1. Jin D J, Gross C A. Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J Mol Biol. 1988;202:45–58. - PubMed
    1. Kunin C M. Antimicrobial activity of rifabutin. Clin Infect Dis. 1996;22(Suppl. 1):S3–S13. - PubMed

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