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. 1976 Feb;13(2):581-9.
doi: 10.1128/iai.13.2.581-589.1976.

Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro

P Anderson, J Pitt, D H Smith

Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro

P Anderson et al. Infect Immun. 1976 Feb.

Abstract

Polyribophosphate, the capsular polysaccharide antigen of Haemophilus influenzae type b, can be assayed in crude bacterial extracts by a combination of immunoprecipitation, hot-acid extraction, and colorimetry. In vitro, the antigen is synthesized during growth and, in well-buffered media, in early stationary phase as well. It is shed from the cell throughout the course of synthesis, and at 37 C almost all the extractable antigen has been released spontaneously within several hours after synthesis. Various strains are similar in extent of synthesis and gross composition of the antigen; however, strains differ in the rate of release during exponential phase. The antigen is released as (or as part of) a very large structure, although under certain culture conditions much smaller antigenic units are found.

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References

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