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. 1976 Jun;33(2):221-32.
doi: 10.1111/j.1365-2141.1976.tb03533.x.

Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease

Z M Ruggeri, P M Mannucci, S L Jeffcoate, G I Ingram

Immunoradiometric assay of factor VIII related antigen, with observations in 32 patients with von Willebrand's disease

Z M Ruggeri et al. Br J Haematol. 1976 Jun.

Abstract

A solid phase non-competitive immunoradiometric assay (IRMA) has been developed which allows measurement of factor VIII related antigen (VIIIR:AG) levels in normal plasma as low as 2.5 x 10(-4) U/ml. The assay is based on the extraction of VIIIR:AG from test plasma by means of polystrene tubes coated with a specific unlabelled anti-VIIIR:AG rabbit antiserum and subsequent labelling of the extracted antigen with 125I-labelled anti-VIIIR:AG rabbit IgG.

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