Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2000 Jun;105(11):1493-9.
doi: 10.1172/JCI10229.

Gut instincts: thoughts on intestinal epithelial stem cells

C Booth  1 , C S Potten
Affiliations
Review

Gut instincts: thoughts on intestinal epithelial stem cells

C Booth et al. J Clin Invest. 2000 Jun.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
A small intestinal crypt (stained with hematoxylin and eosin) with autoradiographic silver grains overlying a single nucleus in the stem-cell region. This label-retaining cell was obtained by administering tritiated thymidine every 6 hours for 48 hours after irradiation with 8 Gy. The sample was taken 8 days later.
Figure 2
Figure 2
(a and b) Examples of “spontaneous” crypt fission (bifurcation) in normal adult mouse small intestine. (c) Multiple fission after irradiation with 12 Gy. A new crypt can be seen developing from the mid-crypt region as well as from the crypt base. Each neocrypt contains Paneth’s cells. (d) Similar bifurcation from a higher crypt-cell position can be seen in the colon after irradiation (10 Gy).
Figure 3
Figure 3
A human small intestinal crypt immunostained for telomerase. Labeling can be seen toward the crypt base, indicating telomere regeneration in the stem cell region.
Figure 4
Figure 4
(a and b) Four-week-old grafts of colonic epithelium derived from isolated crypts. Invaginations similar to those observed during normal crypt development can be seen, with differentiated goblet cells and secreting mucins at the apical pole, and proliferating cells (labeled with tritiated thymidine) at the basal pole. (c) At later times some of these grafts develop a structure more typical of adult intestinal crypts. Adapted from ref. .
See this image and copyright information in PMC

References

    1. Potten, C.S. 1995. Structure, function and proliferative organisation of the mammalian gut. In Radiation and gut. C.S. Potten and J.H. Hendry, editors. Elsevier Science. Amsterdam, The Netherlands. 1–31.
    1. Kaur P, Potten CS. Cell migration velocities in the crypts of the small intestine after cytotoxic insult are not dependent on mitotic activity. Cell Tissue Kinet. 1986;19:601–610. - PubMed
    1. Potten CS, Loeffler M. Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development. 1990;110:1001–1020. - PubMed
    1. Potten CS, Booth C, Pritchard DM. The intestinal epithelial stem cell: the mucosal governor. Int J Exp Pathol. 1997;78:219–243. - PMC - PubMed
    1. Hendry JH, et al. The response of the murine intestinal crypts to short range prometheium-147 radiation. Radiat Res. 1989;118:364–374. - PubMed

Publication types