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. 1976 Nov;32(5):689-93.
doi: 10.1128/aem.32.5.689-693.1976.

Concentration and purification of enteroviruses by membrane chromatography

M Henderson, C Wallis, J L Melnick

Concentration and purification of enteroviruses by membrane chromatography

M Henderson et al. Appl Environ Microbiol. 1976 Nov.

Abstract

A simple procedure for the concentration and partial purification of enteroviruses from tissue culture harvests is described. After removal of acid-precipitating components with a cationic detergent, the detergent and most membrane-coating components were removed by treatment with a cationic-exchange resin. The resin effluent was then acidified, and the virus was adsorbed to epoxy-fiberglass membranes. Virus was then eluted with pH 11.5 glycine-NaOH buffer. Since this eluate contains no orgcentrated simply by acidifying the eluate and passing it through a smaller membrane than that used for the first concentration. As high as 500-fold concentrations can be achieved, with a high efficiency of recovery.

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References

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