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. 2000 May;9(5):1035-7.
doi: 10.1110/ps.9.5.1035.

Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein

S J Blance  1 , N L Williams, Z A Preston, J Bishara, M S Smyth, A Maxwell
Affiliations

Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein

S J Blance et al. Protein Sci. 2000 May.

Abstract

Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.

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