Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2000 Jul;182(13):3843-5.
doi: 10.1128/JB.182.13.3843-3845.2000.

Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects

G Jander  1 , L G Rahme, F M Ausubel
Affiliations

Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects

G Jander et al. J Bacteriol. 2000 Jul.

Abstract

Strain PA14, a human clinical isolate of Pseudomonas aeruginosa, is pathogenic in mice and insects (Galleria mellonella). Analysis of 32 different PA14 mutants in these two hosts showed a novel positive correlation in the virulence patterns. Thus, G. mellonella is a good model system for identifying mammalian virulence factors of P. aeruginosa.

PubMed Disclaimer

Figures

FIG. 1
FIG. 1
G. mellonella 60 h after infection with a mean of one PA14 bacterium. Dead larvae show increased melanization.
FIG. 2
FIG. 2
Time course of PA14 infection in G. mellonella. Twenty-five bacteria per larva were injected at time zero. Each data point represents the mean and standard deviation of bacterial counts from 10 groups of 10 larvae. Larvae died approximately 48 h after injection, when bacterial concentrations reached 109/g.
FIG. 3
FIG. 3
Comparison of LD50 in G. mellonella and percent survival of burned mice after infection with 105 P. aeruginosa bacteria. Solid square, PA14; open squares, individual mutants derived from PA14. y = 17.7 · x + 23.7, P < 0.001, R = 0.565.
See this image and copyright information in PMC

References

    1. Belvin M P, Anderson K V. A conserved signaling pathway: the Drosophila toll-dorsal pathway. Annu Rev Cell Dev Biol. 1996;12:393–416. - PubMed
    1. Bodey G P, Bolivar R. Infections caused by Pseudomonas aeruginosa. Rev Infect Dis. 1983;5:279–313. - PubMed
    1. Boman H G. Peptide antibiotics and their role in innate immunity. Annu Rev Immunol. 1995;13:61–92. - PubMed
    1. Bulla L A, Rhodes R A, St. Julian G. Bacteria as insect pathogens. Annu Rev Microbiol. 1975;29:163–190. - PubMed
    1. Chadwick J S. Serological responses of insects. Fed Proc. 1967;26:1675–1679. - PubMed

Publication types

LinkOut - more resources