[Use of peritoneal macrophages labeled with Cr51 and cultivated in vitro as target cells for quantitative assessment of the cytotoxic activity of immune T-lymphocytes]

Abstract

Peritoneal macrophages previously labeled in a suspension with 51Cr and grown in the form of a discontinuous monolayer on glass, pretreated with poly-l-lysin were used as target cells. This permitted to estimate the cytotoxic activity of immune lymphocytes by 51Cr release for the period of 4 and 20 hours of incubation with the target. 51Cr release from such a target incubated with normal lymphocytes for 20 hours did not exceed 10-20% of the maximum release. Application of a 2% solution of sodium dodecylsulfate ensured a 100 per cent solubilization of labeled macrophages growing on the glass surface, and permitted the cytotoxic effect to be determined by measuring the label remaining in the intact cells.