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. 2000 Jul;44(7):1930-5.
doi: 10.1128/AAC.44.7.1930-1935.2000.

Prevalence of beta-lactamases among 1,072 clinical strains of Proteus mirabilis: a 2-year survey in a French hospital

C Chanal  1 , R Bonnet, C De Champs, D Sirot, R Labia, J Sirot
Affiliations

Prevalence of beta-lactamases among 1,072 clinical strains of Proteus mirabilis: a 2-year survey in a French hospital

C Chanal et al. Antimicrob Agents Chemother. 2000 Jul.

Abstract

beta-Lactam resistance was studied in 1,072 consecutive P. mirabilis clinical strains isolated at the Clermont-Ferrand teaching hospital between April 1996 and March 1998. The frequency of amoxicillin resistance was 48.5%. Among the 520 amoxicillin-resistant isolates, three resistance phenotypes were detected: penicillinase (407 strains [78.3%]), extended-spectrum beta-lactamase (74 strains [14. 2%]), and inhibitor resistance (39 strains [7.5%]). The penicillinase phenotype isolates were divided into three groups according to the level of resistance to beta-lactams, which was shown to be related to the strength of the promoter. The characterization of the different beta-lactamases showed that amoxicillin resistance in P. mirabilis was almost always (97%) associated with TEM or TEM-derived beta-lactamases, most of which evolved via TEM-2.

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Figures

FIG. 1
FIG. 1
Strategy for amplification and/or sequencing of blaTEM genes. Arrows, primers (5′ to 3′). Numbering of amino acids is as described by Ambler et al. (1). Numbering of nucleotides is as described by Sutcliffe (35).
See this image and copyright information in PMC

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