Differing mechanisms of tolerance and desensitization to dinitrochlorobenzene in guinea pigs

Abstract

In the present paper the mechanisms of tolerance and desensitization to dinitrochlorobenzene (DNCB) contact sensitivity in guinea pigs were investigated using the methods of adoptive sensitization of tolerant and normal syngeneic recipients and cyclophosphamide-treatment of tolerant animals known to selectively inactivate suppressor lymphocytes. It was shown that desensitization of presensitized animals is caused by the direct effect of the intravenously injected hapten on the effector cells in the peripheral compartment. The immediate onset of unresponsiveness and its very short duration almost exclude the possible involvement of enhancing antibodies or suppressor cells. In the case of tolerance induced by pretreatment with dinitrobenzenesulfonic acid, suppressor cell activity is enhanced, preventing normal specific immunocompetent cells from recognizing the antigen and/or proliferating in the draining lymph nodes. Whether suppressor lymphocytes are of the B or T type is not yet known.