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Comparative Study
. 1976 Oct;20(1):319-23.
doi: 10.1128/JVI.20.1.319-323.1976.

Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus

J D Gangemi, D G Sharp
Comparative Study

Use of a restriction endonuclease in analyzing the genomes from two different strains of vaccinia virus

J D Gangemi et al. J Virol. 1976 Oct.

Abstract

A restriction endonuclease from Haemophilus influenzae (Hind III) specifically cleaved vaccinia DNA into 14 fragments. The molecular weights of these fragments were determined by gel electrophoresis and ranged from 0.5 x 10(6) to 30 x 10(6). Hind III digestion of the DNA from the WR and CV-1 strains of vaccinia revealed a small molecular difference in one of the resulting fragments. The average molecular weight of the entire vaccinia genome was calculated to be 125 x 10(6).

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