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. 1976;200(3):251-5.
doi: 10.1007/BF01028541.

Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury

A Bolková, J Obenberger

Changes of activity of alkaline and acid phosphatase in the rabbit eye in the early phase of alkaline and acid injury

A Bolková et al. Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1976.

Abstract

Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.

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