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. 1976 Nov 2;144(5):1381-5.
doi: 10.1084/jem.144.5.1381.

Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose

U Hellström, M L Dillner, S Hammarström, P Perlmann

Fractionation of human T lymphocytes on wheat germ agglutinin-sepharose

U Hellström et al. J Exp Med. .

Abstract

T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, convalently bound to Sepharose 6MB. The cells responding to the mitogens leukoagglutinin from Phaseolus vulgaris and concanavalin A were enriched in the strongly binding subpopulation (approximately 20% of the T cells) while they were depleted from the weakly binding subpopulation.

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