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. 1983;2(8):1271-4.
doi: 10.1002/j.1460-2075.1983.tb01580.x.

72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli

B von Wilcken-Bergmann  1 , M Koenen, H W Griesser, B Müller-Hill
Affiliations

72 residues of gal repressor fused to beta-galactosidase repress the gal operon of E. coli

B von Wilcken-Bergmann et al. EMBO J. 1983.

Abstract

An active gene has been constructed which produces a chimera consisting of the N-terminal domain of the gal repressor and all but the first five residues of beta-galactosidase. Seventy two residues of gal repressor fused to beta-galactosidase as tetrameric core are sufficient to repress the gal operon in vivo and to bind to the gal operator in vitro.

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References

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