Altered patterns of innervation in frog muscle after denervation

Abstract

The pattern of reinnervation of muscle fibres after a nerve crush was examined in the cutaneous pectoris muscle of the frog by microscopy and electrophysiology. Normally, about 16% of the muscle fibres are innervated by more than one motor neuron. Two months after reinnervation, about 50% of the fibres are polyneuronally innervated and this high incidence persists for at least seven months. The total number of neurons reinnervating the muscle, as well as the number of muscle fibres comprising the muscle, are normal. However, nerve fibres sprout branches at the site of the crush, and, therefore, the number of axons entering the muscle is greater than normal. Regenerating axons contact muscle fibres precisely at the original synaptic sites and the terminal branches from different axons that end on the same muscle fibre often run side by side occupying stretches or original postsynaptic membrane normally covered by one terminal. Our findings indicate that the amount of synaptic contact during regeneration is limited by the amount of original postsynaptic membrane and that any number of axons that reach vacant portions of original postsynaptic membrane can make synaptic contact with it. Surprisingly, control cutaneous pectoris muscles, situated contralaterally to those that were denervated, also exhibited an abnormal pattern of innervation. Although neither nerve nor muscle was disturbed by the operation, there was a higher incidence of polyneuronal innervation (27% vs 16%) than in muscles of normal animals.