[Studies of improving the frequency of wheat transformation by biolistic bombardment] [In Process Citation]

Abstract

In the present study immature embryos of spring wheat Zhong-60634 were bombarded with gold particles coated with pAHC25 containing Bar and GUS genes. A total of 17 bialaphos-tolerant plants were obtained from 342 immature embryos which were first cultured for 3-4 days and then bombarded and selected. Integration of the introduced genes into the genome of transgenic wheat plants was shown by PCR and Southern analysis and transformation frequency was 0.88%. Furthermore, the present study showed that calli regeneration ability was improved while the embryo culture medium was supplemented with 2 mg/L dicamba instead of 2 mg/L 2,4-D. Meanwhile, in order to keep the calli differentiation ability calli should be transferred to differentiation medium supplemented with 3 mg/L bialaphos from calli selection medium with 5 mg/L bialaphos within a month.