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. 2000 Jul;38(7):2791-2.
doi: 10.1128/JCM.38.7.2791-2792.2000.

Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains

J E Corkill  1 , R Graham, C A Hart, S Stubbs
Affiliations

Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains

J E Corkill et al. J Clin Microbiol. 2000 Jul.
No abstract available

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Figures

FIG. 1
FIG. 1
PFGE patterns obtained with SmaI-digested DNA from C. difficile isolates of known PCR ribotype. Electrophoresis was performed in the absence (a) and in the presence (b) of 50 μM thiourea. Lanes 1 to 5, five degradation-sensitive PCR ribotype 1 isolates (from the United States and Newcastle, Wrexham, Tooting, and Truro, United Kingdom, respectively); lane 6, ribotype 2 isolate (Wrexham); lane 7, ribotype 10 isolate (Manchester, United Kingdom); lane 8, ribotype 15 isolate (Carshalton, United Kingdom); lane 9, ribotype 23 isolate (Jersey, United Kingdom); lane 10, λ DNA standard (48.5-kbp concatemer).
See this image and copyright information in PMC

References

    1. Blaschek H P, Klacik M A. Role of DNase in recovery of plasmid DNA from Clostridium perfringens. Appl Environ Microbiol. 1984;48:78–181. - PMC - PubMed
    1. Brazier J S. The epidemiology and typing of Clostridium difficile. J Antimicrob Chemother. 1998;41(Suppl.):47–57. - PubMed
    1. George W L, Sutter V L, Finegold S M. Antimicrobial agent-induced diarrhoea—a bacterial disease. J Infect Dis. 1977;136:822–828. - PubMed
    1. Hyett A P, Brazier J S, O'Neill G L. Pulsed-field gel electrophoresis as a method for typing Clostridium difficile in the routine laboratory. Rev Med Microbiol. 1997;8(Suppl. 1):S63–S64.
    1. Kato H, Kato N, Watanabe K, Ueno K, Sakata Y, Fujita K. Relapses or reinfections: analysis of a case of Clostridium difficile-associated colitis by two typing systems. Curr Microbiol. 1996;33:220–223. - PubMed

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