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. 2000 Jul 15;349(Pt 2):413-5.
doi: 10.1042/0264-6021:3490413.

Ascorbate oxidation is a prerequisite for its transport into rat liver microsomal vesicles

M Csala  1 , V Mile, A Benedetti, J Mandl, G Bánhegyi
Affiliations

Ascorbate oxidation is a prerequisite for its transport into rat liver microsomal vesicles

M Csala et al. Biochem J. .

Abstract

Oxidation and uptake of ascorbate show similar time courses in rat liver microsomal vesicles: a rapid burst phase is followed by a slower process. Inhibitors of ascorbate oxidation (proadifen, econazole or quercetin) also effectively decreased the uptake of ascorbate. The results show that dehydroascorbate is the transport form of ascorbate at the membrane of the endoplasmic reticulum.

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